A description of the Plasmodium berghei mouse model of malaria and comparisons between features of P. berghei and the human malaria parasite Plasmodium falciparum
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- General introduction to rodent malaria parasites
- Introduction to Plasmodium berghei
- Life cycle of Plasmodium berghei
- Plasmodium berghei infections in vivo
- Morphology of life cycle stages of Plasmodium berghei
- The genome of Plasmodium berghei
– Link to PDF of Plasmodium berghei-Wikipedia page (2020)
– Contribution from Leiden studies
Links to protocols and papers describing technologies used to study mouse models of malaria
from Dr. Chris J. Janse (head of the Leiden Malaria Research Group, 2008-2023)
– Protocols: Leiden parasitological methods – P. berghei
– Paper: P. berghei model (1995)
– Paper: In vitro cultivation of P. berghei ookinetes (1985)
– Paper: Flow cytometry in malaria detection (1994)– Information rodent malaria parasite strains and isolates repository University Edinburgh
– General information P. berghei strains and isolates – genotypes and phenotypes
– Information biosafety transgenic mouse malaria
– Paper: Genome sequences and transcriptomes of rodent malaria parasites (2014)– Transfection protocol – Amaxa drug-selection (paper 2006)
– Transfection protocol – Amaxa flow-selection (paper 2006)
– Movie: protocol isolation of P. berghei schizonts (2006)
– Movie: Protocol transfection Amaxa P. berghei (2006)
– Paper: GIMO transfection rodent malaria parasites (2011)
– Paper: Generation of chimeric rodent malaria parasites expressing human malaria parasite proteins (2015)– Leiden Standard plasmids P. berghei transfection
– Protocol Real-time in vivo imaging of luciferase-expressing P. berghei (paper 2006)
– Luminescence imaging and analysis P. berghei blood-stages (paper 2013)
– Luminescence imaging and analysis P. berghei liver-stages (paper 2013)
– Luminescence imaging and analysis sequestration of P. berghei-infected erythrocytes (paper 2013)– The use of transgenic parasites in malaria vaccine research
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